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SAB4200849 Sigma-Aldrich

Anti-Proteus vulgaris antibody, Mouse monoclonal

clone P.vul-129, purified from hybridoma cell culture

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Properties

Related Categories Antibodies, Microbiome Antibodies More...
antibody form   purified from hybridoma cell culture
antibody product type   primary antibodies
clone   P.vul-129, monoclonal
description   Research area: Microbiome
packaging   antibody small pack of 25 μL
concentration   ~1 mg/mL
application(s)   immunoblotting: 0.005-0.01 μg/mL using dead P. vulgaris bacteria lysate.
isotype   IgG1
shipped in   dry ice
storage temp.   −20°C

Description

General description

Proteus vulgaris is a Gram negative rod-shaped bacteria, belongs to the Enterobacteriaceae family. Members of the genus Proteus (Proteus spp.) which also includes Proteus mirabilis, Proteus penneri and Proteus hauseri, originally characterize by their ability to swarm on solid surfaces, are widespread in the environment and the gastrointestinal tract of human and animals and known to be an opportunistic pathogens isolated from urine, wounds and other clinical sources. The Proteus spp. bacteria, are distinguished by their reactions for indole production, salicin fermentation and aesculin hydrolysis.1-3 P. vulgaris produces indole which differentiates it from the indole-negative P. mirabilis and P. penneri.1-3 Proteus spp. bacteria may also be found in soil or water habitats where they often regarded as indicators of fecal pollution and a contamination threat for potential water or seafood poisoning.1

Specificity

Monoclonal Anti-Proteus vulgaris specifically recognizes P. vulgaris whole extract, the antibody has no cross reactivity with whole extract of Proteus mirabilis, P. gingivalis, E. coli, Pseudomonas aeruginosa, Shigella flexneri, Staphylococcus aureus or Salmonella enterica.

Immunogen

Proteus vulgaris dead OX19 bacteria: ATCC strain 6380

Application

The antibody is recommended to be used in various immunological techniques, including Immunoblot (unidentified protein at ~70kDa). Detection of the P. vulgaris band by Immunoblotting is specifically inhibited by the immunogen.

Biochem/physiol Actions

In the Proteus spp. group, P. mirabilis is encountered in the community and causes the majority of urinary tract Proteus spp. infections whereas P. vulgaris and P. penneri are less common and mainly associated with nosocomial none urinary infections.2,4P. vulgaris have a number of putative virulence factors, including the secreted hemolytic haemolysin and urease, which has been suggested to contribute to host cell invasion and cytotoxicity and bacterial ability to invade uroepithelial cells.4 P. vulgaris, P. mirabilis and P. penneri harbor resistance to ß-lactam antibiotics as it is capable of producing inducible b−lactamases that hydrolyze primary and extended-spectrum penicillins and cephalosporins. 5-6

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

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