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SAB4300195 Sigma-Aldrich

Anti-phospho-MAPK3 (pTyr204) antibody produced in rabbit

affinity isolated antibody

Synonym: Anti-mitogen-activated protein kinase 3 antibody produced in rabbit, Anti-ERK1 antibody produced in rabbit, Anti-HS44KDAP antibody produced in rabbit, Anti-HUMKER1A antibody produced in rabbit, Anti-MGC20180 antibody produced in rabbit

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphoproteins at Phosphotyrosine (pTyr),
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100
  indirect immunofluorescence: 1:100-1:200
  western blot: 1:500-1:1000
species reactivity   rat, mouse, human
mol wt   ~42 kDa
  ~44 kDa
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
concentration   1 mg/mL
immunogen sequence   (T-E-YP-V-A)
NCBI accession no.   NP_001035145.1
UniProt accession no.   P27361
Gene Information   human ... MAPK3(5595)

Description

Immunogen

Peptide sequence around phosphorylation site of tyrosine 204 (T-E-Y(p)-V-A), according to the protein MAPK3.

Target description

Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1.

Physical form

Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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