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  • SAB4300433 - Anti-SRC (Ab-529) antibody produced in rabbit

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SAB4300433 Sigma-Aldrich

Anti-SRC (Ab-529) antibody produced in rabbit

affinity isolated antibody

Synonym: Anti-ASV antibody produced in rabbit, Anti-SRC1 antibody produced in rabbit, Anti-c-SRC antibody produced in rabbit, Anti-p60-Src antibody produced in rabbit, Anti-v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) antibody produced in rabbit

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SP-SS
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100
  western blot: 1:500-1:1000
species reactivity   mouse, rat, human
mol wt   ~60 kDa
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
isotype   IgG
antibody product type   primary antibodies
concentration   1 mg/mL
immunogen sequence   (P-Q-Y-Q-P)
NCBI accession no.   NP_005408.1
UniProt accession no.   P12931
Gene Information   human ... SRC(6714)

Description

Immunogen

Peptide sequence around aa. 527-531 (P-Q-Y-Q-P), according to the protein SRC.

Target description

proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme.

Physical form

Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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