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SAB4300556 Sigma-Aldrich

Anti-SHC1 (Ab-349) antibody produced in rabbit

affinity isolated antibody

Synonym: Anti-FLJ26504 antibody produced in rabbit, Anti-SHC (Src homology 2 domain containing) transforming protein 1 antibody produced in rabbit, Anti-SHC antibody produced in rabbit, Anti-SHCA antibody produced in rabbit

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SG-SK
species reactivity   mouse, rat, human
application(s)   indirect immunofluorescence: 1:100-1:200
  western blot: 1:500-1:1000
clone   polyclonal
concentration   1 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
isotype   IgG
immunogen sequence   (H-Q-Y-Y-N)
mol wt   mol wt ~46 kDa
  mol wt ~52 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... SHC1(6464)
biological source   rabbit
antibody product type   primary antibodies
conjugate   unconjugated
NCBI accession no.   NP_001123512.1
UniProt accession no.   P29353

Description

Immunogen

Peptide sequence around aa. 347-351 (H-Q-Y-Y-N), according to the protein SHC1.

Physical form

Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Target description

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span.

Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  1
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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