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SAB4300705 Sigma-Aldrich

Anti-REST antibody produced in rabbit

affinity isolated antibody

Synonym: Anti-NRSF, Anti-REST, Anti-XBR

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, RB-RG
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 1:500-1:1000
species reactivity   human
mol wt   ~121 kDa
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
concentration   1 mg/mL
immunogen sequence   (R-E-E-A-S)
NCBI accession no.   NM_001193508.1
UniProt accession no.   Q13127
Gene Information   human ... REST(5978)

Description

General description

REST (RE1 silencing transcription factor) is a transcriptional repressor, which is also termed as NRSF (neuron-restrictive silencer factor).{26,29} It is abundantly present in the body. The gene is located on human chromosome 4q12 (5).

The antibody detects endogenous level of total REST protein.

Immunogen

Peptide sequence around aa. 873-877 (R-E-E-A-S), according to the protein NP_001180437.1

Target description

Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.

Physical form

Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

REST (RE1 silencing transcription factor) plays a major role in hematopoietic and cardiac differentiation. Mutations in REST lead to hereditary gingival fibromatosis. It serves as a tumor suppressor in breast cancer. In human embryonic stem cells, suppression of REST/NRSF affects their survival, lineage differentiation and signaling.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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