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  • SAB4301243 - Anti-phospho-ACK1 (pTyr284) antibody produced in rabbit

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SAB4301243 Sigma-Aldrich

Anti-phospho-ACK1 (pTyr284) antibody produced in rabbit

affinity isolated antibody

Synonym: ACK1, Activated p21cdc42Hs kinase, EC 2.7.10.2, kinase ACK1

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphoproteins at Phosphotyrosine (pTyr),
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: 1:100-1:200
  immunohistochemistry: 1:50-1:100
  western blot: 1:500-1:1000 (Cell Lysate)
species reactivity   mouse, human
mol wt   mol wt 120 kDa
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
isotype   IgG
antibody product type   primary antibodies
concentration   1.0 mg/mL
accession no.   NP_005772.3.
UniProt accession no.   Q07912
Gene Information   human ... TNK2(10188)

Description

Specificity

The antibody detects endogenous levels of ACK1 only when phosphorylated at tyrosine 284.

Immunogen

Peptide sequence around phosphorylation site of tyrosine 284 (D-H-Y(p)-V-M) derived from Human ACK1.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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