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  • SAB4301419 - Anti-phospho-NF-κB p100 (pSer872) antibody produced in rabbit

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SAB4301419 Sigma-Aldrich

Anti-phospho-NF-κB p100 (pSer872) antibody produced in rabbit

affinity isolated antibody

Synonym: DNA-binding factor KBF2, Lymphocyte translocation chromosome 10, Lyt10, Nuclear factor NF-kappa-B p100 subunit, Oncogene Lyt-10

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphoproteins at Phosphoserine (pSer),
clone   polyclonal
biological source   rabbit
application(s)   western blot: 1:500-1:1000 (Cell Lysate)
species reactivity   mouse, human, rat
mol wt   100 kDa
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
concentration   1.0 mg/mL
accession no.   NP_001070962.1
UniProt accession no.   Q00653
Gene Information   human ... NFKB2(4791)

Description

General description

Nuclear factor kappa B subunit 2 (NFKB2) encodes the nuclear factor kappa B2 (NF-κB), a nuclear transcription factor. NF-κB is evolutionarily highly conserved. In human chromosome, the gene NFKB2 is localized on 10q24.

Specificity

The antibody detects endogenous level of NF-κB p100 only when phosphorylated at serine 872.

Immunogen

Peptide sequence around phosphorylation site of serine872(S-Q-S(p)-V-E) derived from Human NF-κB p100 .

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Regulation of nuclear factor kappa B subunit 2 (NFKB2) includes cytoplasmic-nuclear shuttling and its transcriptional activity. NF-κB plays a key role in regulating the immune response and apoptotic processes. It is necessary for combating diseases like arthritis, autoimmune diseases and asthma. Mutations in NFKB2 impairs post translational processing of p100 preventing nuclear translocation of p52. This leads to common variable immunodeficiency and adrenal insufficiency. Mutation also affects canonical and non-canonical pathway of NFKB2 signalling and causes autosomal dominant primary antibody deficiency (PAD). chromosomal aberrations in C-terminal region of NFKB2 is associated lymphoid malignancies.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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