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SAB4502249 Sigma-Aldrich

Anti-Keratin 20 antibody produced in rabbit

affinity isolated antibody

Synonym: CK-20, Cytokeratin-20, K20, Keratin type I cytoskeletal 20

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Properties

Related Categories Alphabetical Index, Antibodies, K-KH, Primary Antibodies
species reactivity   mouse, rat, human
application(s)   ELISA: 1:10000
  immunohistochemistry: 1:50-1:100
  western blot: 1:500-1:1000
clone   polyclonal
concentration   ~1 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 48 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... KRT20(54474)
biological source   rabbit
antibody product type   primary antibodies
conjugate   unconjugated
NCBI accession no.   P35900
UniProt accession no.   P35900

Description

Immunogen

The antiserum was produced against synthesized peptide derived from human Keratin 20.

Immunogen Range: 375-424

General description

Anti-Keratin 20 Antibody detects endogenous levels of total Keratin 20 protein.
The gene KRT20 (keratin 20) is mapped to human chromosome 17. The gene encodes an intermediate filament which is mainly expressed in the gastrointestinal epithelial cells and epidermal Merkel cells. The protein is a type 1 keratin.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

KRT20 (keratin 20) helps in intermediate filament organization. In metastatic colorectal cancer, it can be used as a biomarker for checking circulating tumor cells.

Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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