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SAB4502834 Sigma-Aldrich

Anti-SOX9 antibody produced in rabbit

affinity isolated antibody

Synonym: SRY sex determining region Y-box 9 protein, SRY-box containing gene 9, Sox-9, transcription factor SOX-9

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SO-SO
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   ELISA: 1:5000
  immunofluorescence: 1:100-1:500
  immunohistochemistry: 1:50-1:100
  western blot: 1:500-1:1000
species reactivity   mouse, human
mol wt   antigen 56 kDa
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   ~1 mg/mL
NCBI accession no.   P48436
UniProt accession no.   P48436
Gene Information   human ... SOX9(6662)

Description

General description

Anti-SOX9 antibody detects endogenous levels of total SOX9 protein.

The SOX9 (SRY (sex determining region Y)-box 9) gene is located on human chromosome 17q24.3. SOX9 codes for a DNA-binding protein that belongs to the class of high-mobility-group box.

Immunogen

The antiserum was produced against synthesized peptide derived from human SOX9.

Immunogen Range: 147-196

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The protein encoded by SOX9 (SRY (sex determining region Y)-box 9) gene is involved in the regulation of developmental processes such as chondrogenesis, gliogenesis, epithelial hair follicle stem cell specification, gonadogenesis, normal development of septum, lung, cardiac valve and pancreas. Abnormal regulation of SOX9 promotes tumorigenesis in different tissues. SOX9 induces the process of metastasis in cancer cells. Overexpression of the gene is observed in prostate, breast, and colorectal cancers.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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