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SAB4503247 Sigma-Aldrich

Anti-AKAP1 antibody produced in rabbit

affinity isolated antibody

Synonym: A kinase anchor protein 1, A-kinase anchor protein 149 kDa, AKAP 149, AKAP149, mitochondrial precursor

  •  NACRES NA.41

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Properties

Related Categories AE-AK, Alphabetical Index, Antibodies, Primary Antibodies
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   ELISA: 1:1000
  immunofluorescence: 1:100-1:500
  immunohistochemistry: 1:50-1:100
  western blot: 1:500-1:1000
species reactivity   human
mol wt   antigen 97 kDa
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   100
antibody product type   primary antibodies
concentration   ~1 mg/mL
NCBI accession no.   Q92667
UniProt accession no.   Q92667
Gene Information   human ... AKAP1(8165)

Description

General description

The AKAP1 (A-kinase anchoring protein 1) gene is mapped to human chromosome 17q22. This gene encodes a scaffolding protein localized to the mitochondria. Anti-AKAP1 antibody detects endogenous levels of total AKAP1 protein.

Immunogen

The antiserum was produced against synthesized peptide derived from human AKAP1.

Immunogen Range: 281-330

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Biochem/physiol Actions

AKAP1 (A-kinase anchoring protein 1) controls organelle formation and oxidative metabolism. It is essential for cell survival. It is responsible for anchoring signaling molecules such as protein kinase A to the outer mitochondrial membrane. AKAP1 is associated with the process of tumorigenesis, by mediating mammalian target of rapamycin (mTOR) pathway. Thus, reduced expression of AKAP1 affects mTOR pathway and prevents glioblastoma growth.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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