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  • SAB4503754 - Anti-phospho-AMPK α (pThr172) antibody produced in rabbit

SAB4503754 Sigma-Aldrich

Anti-phospho-AMPK α (pThr172) antibody produced in rabbit

affinity isolated antibody



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphoproteins at Phosphothreonine (pThr),
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   ELISA: 1:40000
  immunohistochemistry: 1:50-1:100
  western blot: 1:500-1:1000
species reactivity   rat, human, mouse
mol wt   antigen 62 kDa
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   ~1 mg/mL
NCBI accession no.   Q13131
UniProt accession no.   P54646
Gene Information   human ... PRKAA1(5562), PRKAA2(5563)


General description

AMP-activated protein kinase α (AMPKα) exists in two isoforms α1 and α2. The gene coding for AMPK α1 and AMPKα2 is mapped to human chromosome 5p11-p14 and 1p31 respectively. AMPKα is a 63kDa catalytic subunit of AMPK, involved in regulation of AMP levels. The AMP-activated protein kinase (AMPK) is a ubiquitously expressed cellular energy sensor.


The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172.

Immunogen Range: 140-189


Anti-phospho-AMPKα (pThr172) antibody produced in rabbit has been used in immunohistochemistry (IHC) staining.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

AMP-activated protein kinase is activated by the increased concentration of adenosine mono phosphate (AMP) during mitochondrial dysfunction. AMPK, or AMPK α1, is a vital constituent of the adaptive response to cardiomyocyte stress that occurs during ischemia. AMPK is involved in regulation of various cellular processes such as mitochondrial biogenesis, autophagy and inflammation, and immune responses. AMPK signaling pathway aids in maintaining energy balance in retinal neurons, therefore, it is considered as a potential therapeutic target to prevent or delay retinal degeneration. AMPK acts as a cellular energy sensor that controls glucose and lipid metabolism in response to change in nutrient and intracellular energy levels. C allele of protein kinase AMP-activated catalytic subunit α 1 (PRKAA1) rs13361707 is associated with susceptibility of gastric cancer (GCa). AMPKα2 plays an essential role in controlling postmortem glycolysis in skeletal muscle.

Features and Benefits

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Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

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Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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