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  • SAB4700050 - Monoclonal Anti-CD3 antibody produced in mouse

SAB4700050 Sigma-Aldrich

Monoclonal Anti-CD3 antibody produced in mouse

clone APA1/1, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-CD3 (activation epitope)

  •  NACRES NA.41



Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Flow Cytometry, Antibodies to CD and Related Antigens,
conjugate   unconjugated
clone   APA1/1, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
species reactivity   mouse, human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
concentration   1 mg/mL
NCBI accession no.   NM_000733.3
Gene Information   human ... CD3(916)


General description

CD3 (cluster of differentiation 3) molecule is encoded by the gene mapped to human chromosome 11q23.3. The encoded protein exists in three isoforms, CD3ε, CD3γ and CD3δ and each contains an N-terminal extracellular domain, a transmembrane segment and a cytoplasmic domain. CD3 is a 20kDa glycoprotein expressed on surface of all human T lymphocytes.


Purified human CD3 proteins isolated from thymus


The reagent is designed for Flow Cytometry analysis. Suggested working dilution for Flow Cytometry is 1-2 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Physical form

Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

T-cell receptor- CD3 (cluster of differentiation 3) complex including CD3ε, CD3γ and CD3δ, plays a vital role in inducing early metabolic events that lead to T cell activation. Mutation in CD3ε has been observed in T-B+ NK+ severe combined immunodeficiency (SCID) patients.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Safety & Documentation

Safety Information

NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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