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SAB4700276 Sigma-Aldrich

Monoclonal Anti-CD16 antibody produced in mouse

clone MEM-154, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-FCGR3A, Anti-FcγRIII

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Flow Cytometry, Antibodies to CD and Related Antigens, FB-FJ,
conjugate   unconjugated
clone   MEM-154, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
concentration   1 mg/mL
NCBI accession no.   NM_000569.6
UniProt accession no.   P08637
Gene Information   human ... FCGR3A(2214)

Description

General description

The antibody MEM-154 reacts with the epitope on CD16 antigen that residing in proximity to FG loop (probably BC or C′E loop). CD16 is a low affinity receptor for aggregated IgG (FcgammaRIII antigen). The antibody MEM-154 reacts with CD16+ granulocytes.

Immunogen

Human granulocytes

Application

The reagent is designed for Flow Cytometry analysis of human blood cells using 5-10 μg/mL of sample.

Physical form

Solution in Tris buffered saline, pH 8.0, with 15 mM sodium azide.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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