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SAB4700346 Sigma-Aldrich

Monoclonal Anti-IgM antibody produced in mouse

clone CH2, purified immunoglobulin, buffered aqueous solution

  •  NACRES NA.46

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Flow Cytometry, Antibodies to Immunoglobulins, I-IG,
conjugate   unconjugated
clone   CH2, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
antibody form   purified immunoglobulin
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
concentration   1 mg/mL
NCBI accession no.   NC_000014.8
Gene Information   human ... IGH@(3492)

Description

General description

Immunoglobulin M (IgM) is a member of immunoglobulin family. It exists as a pentamer in the serum and has a high molecular weight. Monomeric IgM has a molecular weight of 190 kDa. The pentameric IgM is characterized with ten μ heavy chains, ten light chains and a single J-chain which is linked by disulphide bonds. The μ chain consists of five domains- VH, Cμ1, Cμ2, Cμ3 and Cμ4. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as fragment antigen binding (Fab) fragments. The third fragment does not possess antigen-binding activity and is known as fragment crystallizable (Fc). It interacts with cells and effector molecules. The heavy chains of IgM contain an extra C domain that replaces the hinge region. IgM is the first antibody to be produced during an immune response and is the major isotype secreted in T-cell independent immune responses.

The antibody CH2 reacts with Fc fragment of human IgM.

Immunogen

Purified human IgM

Application

The reagent is designed for Flow Cytometry analysis. Suggested working dilution for Flow Cytometry is 1 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Physical form

Solution in Tris buffered saline, pH 8.0, with 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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