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SAE0010 Sigma-Aldrich

EC-Oxyrase®

Synonym: EC-Oxyrase®

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Properties

Related Categories Base Ingredients for Media, Cell Culture, ITS and Other Supplements, Microbiology, Other Additives,
shipped in   dry ice
storage temp.   −20°C
  −20°C

Description

Analysis Note

EC Oxyrase contains a penicillin binding protein that may interfere with penicillin and some related antibiotics.

Application

Oxyrase is an enzyme system that offers a way to remove dissolved oxygen from liquid, gas or semisolid products. Oxyrase utilizes the same mechanics of respiration employed by all living beings. It selectively captures oxygen and with a substrate converts it to water. Oxyrase is equivalent to a “strong” reducing agent, but unlike chemical antioxidants, Oxyrase, the substrates and reactants are all-natural. Oxyrase is designed to work efficiently over a wide range of pH and temperature

Various applications of Oxyrase include the preparation of anaerobic media for culture of anaerobic microorganisms, and the protection of oxygen-sensitive materials. In studies of biological processes, Oxyrase can help to enhance the intensity and longevity of activated fluorescent dye which are at risk of oxygen quenching.

EC-Oxyrase® has been used as a component of imaging buffer for stochastic optical reconstruction microscopy (STORM) imaging.

Biochem/physiol Actions

EC-Oxyrase is active over a wide temperature range. It can be kept at 40 °C for hours without substantial loss of activity. It is heat inactivated above 55°C.

EC-Oxyrase operates over a wide pH range of 6.8 to 9.4. The optimum pH is about 8.0. As the pH moves away from optimum, activity decreases. The lower activity level can be compensated for by increasing time to complete oxygen removal or by increasing EC-Oxyrase concentration.

EC-Oxyrase requires substrate to be active. Levels at 10 mM to 20 mM are adequate. Substrates for EC Oxyrase include lactic acid, succinic acid, formic acid or their salts, and alpha-glycerol phosphate inaddition to oxygen. EC Oxyrase also contains L-sodium lactate at 100 mM to stabilize the Oxyrase during freeze-thaw cycles. This amount of substrate is diluted to extinction upon use.

The exact volume of EC Oxyrase and substrates needed to reduce oxygen in a given system are determined by a number of parameters: including pH, temperature, kinds and amounts of substrates present, surface to depth ratio of the container, and headspace volume. Some experimentation may be necessary; a suggested use level is a 1:100 dilution of the product.

General description

The Oxyrase Enzyme System is obtained from the cytoplasmic membrane of E, coli

Legal Information

Oxyrase is a registered trademark of Oxyrase, Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin


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15

References

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