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SAE0064 Sigma-Aldrich

Angiotensin Converting Enzyme-2, ACE2

Human recombinant, ≥1000000 U/mg, expressed in HEK 293 cells

Synonym: ACE-related carboxypeptidase, ACE2, Angiotensin-converting enzyme homolog (ACEH), COVID-19 receptor, Coronavirus receptor, Metalloprotease MPROT15

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Properties

Related Categories Angiotensin Converting Enzyme, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Proteolytic Enzymes and Substrates, Selective Proteolytic Enzymes More...
Quality Level   200
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
mol wt   ~85.9 kDa by SDS-PAGE
impurities   ≤1 EU/μg protein Endotoxin
shipped in   ambient
storage temp.   −20°C

Description

General description

Angiotensin Converting Enzyme-2 (ACE-2) is the first known human homologue of angiotensin-converting enzyme (ACE). COVID-19 virus has been shown to use its surface protein S protein to bind to human ACE-2. The S protein is cleaved into subunits, S1 and S2 during the COVID-19 infection. S1 contains the receptor binding domain (RBD) which enables coronaviruses to bind to the peptidase domain (PD) of ACE2 It was identified from 5′ sequencing of a human heart failure ventricle cDNA library. ACE-2 has an apparent signal peptide, a single metalloprotease active site, and a transmembrane domain. ACE-2, like ACE, is a membrane-associated and secreted enzyme expressed predominantly on endothelium, but unlike ACE, it is highly restricted in humans to heart, kidney, and testis.
ACE-2 is a new component of the renin-angiotensin system (RAS). Accumulating evidence shows that Ace-2 provides protective effects in peripheral tissues and has great potential for the treatment of RAS-related diseases. The emerging concept is that an imbalance in Ace-2/Ang-(1-7) and ACE/Ang-II axes is critical in the development of cardiovascular diseases. The central role of ACE-2, therefore, appears to counter ACE activity by reducing Ang-II bioavailability and increasing Ang(1-7) formation.

Biochem/physiol Actions

Recombinant human Angiotensin Converting Enzyme-2 (ACE-2) is expressed in human HEK 293 cells as a C-terminally flag and his-tagged glycoprotein with a calculated molecular mass of 85.9 kDa (amino acids Gln18-Ser740, with a C-terminal 10-His tag). The DTT-reduced protein migrates as a 90-120 kDa polypeptide on SDS-PAGE due to glycosylation. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein.

Unit Definition

One unit is defined as the amount of enzyme required to cleave 1 picomole of the fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH in one minute, in 37 °C, pH 7.5.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

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