EMAIL THIS PAGE TO A FRIEND
SAE0083 Sigma-Aldrich

Auresine New

recombinant, lyophilized powder

  •  EC Index Number 3.4.24.75

Purchase

Properties

recombinant   expressed in Pichia pastoris
form   lyophilized powder
activity   >500 units/mg protein
mol wt   mol wt 14.4 kDa
storage condition   room temperature

Description

Preparation Note

Auresine in soluble in water (20 mg/ml), yielding a clear solution. The product is active in 100 nM concentration (1.4 ug/mL) in low conductivity buffers (= 2 mS/cm) at temp. 0-40°C.

Unit Definition

One unit will reduce the turbidity (A600) of suspension of S. aureus cells from 0.250 to 0.125 in 10 minutes in 50 mM glycine, pH 8.0 at 25°C in 6.0 mL reaction mixture.

Application

Laboratory use (component of kits for isolation of protoplasts, nucleic acids, proteins, lipids and other components of Gram positive bacterial cells. Thanks to the unique activity in non-physiological conditions (low conductivity and low temperatures) the isolated components, like nucleic acids and proteins are not prone to degradation by released cellular enzymes).

General description

Auresine is a portion of the zinc metalloprotease Staphylococcus aureus. The enzyme has glycyl-glycine endopeptidase activity and it specifically cleaves polyglycine crosslinks in the cellular wall of Staphylococcus species, including various strains of methicillin-resistant Staphylococcus aureus (MRSA).

Storage and Stability

Auresine lyophilized powder should be stored at room temperature. Once dissolved in storage buffer, Auresine is stable at room temperature up to 4 months, at 4°C, -20°C, -80°C at least 2 years. Storage buffer: 20 mM Tris-HCl, pH 7.0, 200 mM NaCl, 10% glycerol.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?