SAE0083 Sigma-Aldrich

Auresine New

recombinant, lyophilized powder

  •  EC Index Number



recombinant   expressed in Pichia pastoris
form   lyophilized powder
activity   >500 units/mg protein
mol wt   mol wt 14.4 kDa
storage condition   room temperature


Preparation Note

Auresine in soluble in water (20 mg/ml), yielding a clear solution. The product is active in 100 nM concentration (1.4 ug/mL) in low conductivity buffers (= 2 mS/cm) at temp. 0-40°C.

Unit Definition

One unit will reduce the turbidity (A600) of suspension of S. aureus cells from 0.250 to 0.125 in 10 minutes in 50 mM glycine, pH 8.0 at 25°C in 6.0 mL reaction mixture.


Laboratory use (component of kits for isolation of protoplasts, nucleic acids, proteins, lipids and other components of Gram positive bacterial cells. Thanks to the unique activity in non-physiological conditions (low conductivity and low temperatures) the isolated components, like nucleic acids and proteins are not prone to degradation by released cellular enzymes).

General description

Auresine is a portion of the zinc metalloprotease Staphylococcus aureus. The enzyme has glycyl-glycine endopeptidase activity and it specifically cleaves polyglycine crosslinks in the cellular wall of Staphylococcus species, including various strains of methicillin-resistant Staphylococcus aureus (MRSA).

Storage and Stability

Auresine lyophilized powder should be stored at room temperature. Once dissolved in storage buffer, Auresine is stable at room temperature up to 4 months, at 4°C, -20°C, -80°C at least 2 years. Storage buffer: 20 mM Tris-HCl, pH 7.0, 200 mM NaCl, 10% glycerol.

Safety & Documentation

Safety Information

NONH for all modes of transport
Protocols & Articles
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