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SBPE03 Sigma-Aldrich

SB-MRP2-Sf9-PREDEASY-ATPase kit

membrane based kit for Human MRP2 ATPase Transport Assays

Synonym: ABCC2, MRP2 human membrane based kit for Human MRP2 ATPase Transport Assays, MRP2, Multidrug resistance protein 2

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Properties

Related Categories ADME/Tox Products, Cell Biology, Kits for Transporter Assays, Membrane Preparations for ATPase Assays
recombinant   expressed in insect cells
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... ABCC2(1244)

Description

General description

SOLVO Biotechnology offers ready-to-use assay kits for efflux and uptake transporters. SOLVO′s PREDEASY ATPase Assay Kit provides everything necessary to conduct ATPase activation and inhibition assays to better understand the nature of the interaction between a compound and the transporter. By measuring ATPase activity, both activation and inhibition of transporters can be investigated.

Application

SOLVO′s PREDEASY ATPase Assay Kit is a unique product for measuring the ATPase-dependent drug - ABC membrane transporter interaction in a convenient, "ready-to-assay" package. The ATPase assay is an in vitro membrane assay designed to indicate the nature of the interaction between the compound and the transporter. By measuring ATPase activity, both activation and inhibition of transporters can be investigated using membranes from baculovirus-infected insect cells or mammalian cell membranes containing high levels of human or rodent wild-type transporters. ABC transporters mediate the transport of substrates against a concentration gradient using energy derived from ATP hydrolysis, which is proportional to the transporter activity and could easily be detected with a colorimetric method.

PREDIVEZ PREDEASY Kits contain all reagents (chemicals) and solutions necessary to conduct ATPase activation and inhibition assays. You only have to add water. Each kit comes with a CD that includes the assay protocol, data sheet, SDS, electronic data processing, evaluation templates and the reference data. The recommended layout allows activation and inhibition measurements in duplicates for 8 concentrations of the test article on one 96-well plate with all of the relevant controls.

PREDEASY ATPase Assay Kits are available for 3 and 10 plates for the transporters recommended by the FDA and the EMA.

Physical form

Supplied as frozen membrane vesicles, containing 5 mg/ml membrane protein, labeled with volume, catalog number (transporter), date of production, protocols and necessary reagents sufficient for the analysis of 3 or 10, 96-well plates.

Legal Information

Distributed for SOLVO Biotechnology, Inc.

PREDEASY is a trademark of Solvo Biotechnology

PREDIVEZ is a trademark of Solvo Biotechnology

Safety & Documentation

Safety Information

RIDADR 
UN 1789 8 / PGII

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

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Description

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SBVT14 SB-MRP2-HEK293
SBCT06 SB-HEK293-Mock-CTRL, Negative control membrane for HEK293 transporter membranes
SBVT05 SB-MRP2-Sf9, membrane preparation for Vesicular Transport Assays, recombinant, expressed in baculovirus infected Sf9 cells
SBVT13 SB-ratMrp2-HEK293, MRP2 rat vesicles

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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