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SBPE13 Sigma-Aldrich

SB-mouseBsep-Sf9-HAM-PREDEASY-ATPase kit

Synonym: BSEP, Bile salt export pump, Bsep mouse membrane based kit for Mouse Bsep ATPase Transport Assays, mouse ABCB11

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Properties

Related Categories ADME/Tox Products, Cell Biology, Kits for Transporter Assays, Membrane Preparations for ATPase Assays
recombinant   expressed in insect cells
  expressed in insect cells
form   liquid
shipped in   dry ice
storage temp.   −70°C

Description

General description

SOLVO Biotechnology offers ready-to-use assay kits for efflux and uptake transporters. SOLVO′s PREDEASY ATPase Assay Kit provides everything necessary to conduct ATPase activation and inhibition assays to better understand the nature of the interaction between a compound and the transporter. By measuring ATPase activity, both activation and inhibition of transporters can be investigated.

Application

SOLVO′s PREDEASY ATPase Assay Kit is a unique product for measuring the ATPase-dependent drug - ABC membrane transporter interaction in a convenient, "ready-to-assay" package. The ATPase assay is an in vitro membrane assay designed to indicate the nature of the interaction between the compound and the transporter. By measuring ATPase activity, both activation and inhibition of transporters can be investigated using membranes from baculovirus-infected insect cells or mammalian cell membranes containing high levels of human or rodent wild-type transporters. ABC transporters mediate the transport of substrates against a concentration gradient using energy derived from ATP hydrolysis, which is proportional to the transporter activity and could easily be detected with a colorimetric method.

PREDIVEZ PREDEASY Kits contain all reagents (chemicals) and solutions necessary to conduct ATPase activation and inhibition assays. You only have to add water. Each kit comes with a CD that includes the assay protocol, data sheet, SDS, electronic data processing, evaluation templates and the reference data. The recommended layout allows activation and inhibition measurements in duplicates for 8 concentrations of the test article on one 96-well plate with all of the relevant controls.

PREDEASY ATPase Assay Kits are available for 3 and 10 plates for the transporters recommended by the FDA and the EMA.

Physical form

Supplied as frozen membrane vesicles, containing 5 mg/ml membrane protein, labeled with volume, catalog number (transporter), date of production, protocols and necessary reagents sufficient for the analysis of 3 or 10, 96-well plates.

Legal Information

PREDEASY is a trademark of Solvo Biotechnology

PREDIVEZ is a trademark of Solvo Biotechnology

Safety & Documentation

Safety Information

RIDADR 
UN 3316 9

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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