SBVT09 Sigma-Aldrich


recombinant, expressed in human cells (expressed in proprietary human “M” cells)

Synonym: ABCG2, BCRP human membrane vesicles, BCRP, Breast Cancer Resistance Protein



Related Categories ADME/Tox Products, Cell Biology, Membrane Preparations for Transporter Assays More...
recombinant   expressed in human cells (expressed in proprietary human “M” cells)
form   liquid
concentration   5.0 mg/mL
color   off-white
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... ABCG2(9429)


General description

Membrane Preparations for Vesicular Transport Assays (VT) are suitable for general drug-efflux transporter interaction studies. Both substrate and inhibitor interactions can be assessed using vesicles. The success of substrate interaction studies strongly depends on the passive permeability of the compound. High permeability substrates might not be detected. Control Membranes with no-, or significantly lower transporter activity are also available.


In the vesicular transport assay so-called "inside-out" membrane vesicles containing ABC transporters are applied. Incubating substrates of the respective efflux transporter in the presence of the inverted membrane vesicles and ATP will allow measuring accumulation of the substrates into the vesicles. In many cases radiolabeled reporter substrates are used but recently SOLVO developed the new PREDIVEZ Vesicular Transport Kits that use fluorescent reporter substrates.

The standard vesicular transport assay is an inhibitory assay performed with cold test articles. This assay provides information on any interaction between the ABC transporter and the test article. The transport of the reporter substrate is measured in the presence of the test article (typically in 7 concentrations) and IC50 is defined as the concentration inhibiting the transport of the reporter substrate by 50%.

Should radiolabeled form of the investigated compound or adequate analytical methods (LC/MS, HPLC) be available, the vesicular transport assay may be performed in a direct format without the reporter substrate and may identify substrate nature of the test article. The vesicular transport substrate assay is a low throughput assay. It is suitable for low permeability test compounds as high permeability compounds may escape from the vesicles through the lipid bilayer.

PREDIVEZ vesicular transport kit is sold separately. This transporter membrane vesicle assay requires the PREDIVEZ vesicular transport kit and corresponding control membrane below:

SB PREDIVEZ Reagent Kit for BCRP part number SBPVR4-9RXN and corresponding control membrane SB-M-CTRL part number SBCT02-1EA

Physical form

Supplied as frozen membrane vesicles, containing 5 mg/ml membrane protein, labeled with volume, catalog number (transporter) and date of production.

Legal Information

Distributed for SOLVO Biotechnology, Inc.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers


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SBCT02 SB M CTRL, recombinant, expressed in human cells (expressed in proprietary human “M” cells)
SBPE05 SB-BCRP-M-PREDEASY-ATPase kit, membrane based kit for Human BCRP ATPase Transport Assays

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