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SBVT18 Sigma-Aldrich

SB-BSEP-HEK293

Synonym: ABC16, BRIC2, BSEP human membrane vesicles, BSEP, Bile salt export pump

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Properties

Related Categories ADME/Tox Products, Cell Biology, HEK293-based Membrane Preparations for Transporter Assays, Membrane Preparations for Transporter Assays
form   liquid
concentration   5 mg/mL
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... ABCB11(8647)

Description

General description

Membrane Preparations for Vesicular Transport Assays (VT) are suitable for general drug-efflux transporter interaction studies. Both substrate and inhibitor interactions can be assessed using vesicles. The success of substrate interaction studies strongly depends on the passive permeability of the compound. High permeability substrates might not be detected. Control Membranes with no-, or significantly lower transporter activity are also available.

Application

In the vesicular transport assay so-called "inside-out" membrane vesicles containing ABC transporters are applied. Incubating substrates of the respective efflux transporter in the presence of the inverted membrane vesicles and ATP will allow measuring accumulation of the substrates into the vesicles. In many cases radiolabeled reporter substrates are used but recently SOLVO developed the new PREDIVEZ Vesicular Transport Kits that use fluorescent reporter substrates.

The standard vesicular transport assay is an inhibitory assay performed with cold test articles. This assay provides information on any interaction between the ABC transporter and the test article. The transport of the reporter substrate is measured in the presence of the test article (typically in 7 concentrations) and IC50 is defined as the concentration inhibiting the transport of the reporter substrate by 50%.

Should radiolabeled form of the investigated compound or adequate analytical methods (LC/MS, HPLC) be available, the vesicular transport assay may be performed in a direct format without the reporter substrate and may identify substrate nature of the test article. The vesicular transport substrate assay is a low throughput assay. It is suitable for low permeability test compounds as high permeability compounds may escape from the vesicles through the lipid bilayer.

Packaging

500 μL

Physical form

Supplied as frozen membrane vesicles, containing 5 mg/ml membrane protein, labeled with volume, catalog number (transporter) and date of production.

Legal Information

Distributed for SOLVO Biotechnology, Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Milli-Q® Water Purification Solutions
Protocols & Articles
Peer-Reviewed Papers
15

References

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SBCT06 SB-HEK293-Mock-CTRL, Negative control membrane for HEK293 transporter membranes
SBVT12 SB-mouseBsep-Sf9, membrane preparation for Vesicular Transport Assays, recombinant, expressed in baculovirus infected Sf9 cells
SBVT03 SB-BSEP-Hi5, Membrane preparation for Vesicular Transport Assays, recombinant, expressed in Baculovirus infected High-5 cells
SBVT02 SB-BSEP-Sf9, membrane preparation for Vesicular Transport Assays, recombinant, expressed in baculovirus infected Sf9 cells

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