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SCU0001 Sigma-Aldrich

Interleukin-6 human

recombinant, expressed in HEK 293 cells, suitable for cell culture

Synonym: IL-6

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Properties

Related Categories CNTF Family - includes ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF) and interleukin-6 (IL-6), Cell Culture, Growth Factors and Cytokines, Interleukin-6, Interleukins (IL),
Quality Level   200
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥98% (SDS-PAGE)
form   lyophilized powder
potency   0.2-2 ng/mL EC50
quality   endotoxin tested
mol wt   calculated mol wt 20.8 kDa (183 amino acids glycosylated protein)
packaging   pkg of 10 μg
application(s)   cell culture | mammalian: suitable
impurities   ≤0.1 EU/μg protein (LAL test)
color   white
UniProt accession no.   P05231
storage temp.   −20°C
Gene Information   human ... IL6(3569)

Description

General description

Interleukin 6 (IL-6) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. IL-6 is a multifunctional cytokine with well-defined pro- and anti-inflammatory properties. IL-6 is secreted by T cells and macrophages to stimulate immune response, e.g. during infection and after trauma, especially burns or other tissue damage leading to inflammation. The O-linked sugar chain of IL-6 is thought to contribute to the stability of the cytokine through polymerization suppression and/or conformational changes, exemplifying the significance of its expression in a human expression system (which allows proper glycosylation).

Biochem/physiol Actions

Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β2), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Physical form

This product is supplied as a lyophilized powder in phosphate buffered saline (PBS), pH 7.4, with no additives or carrier proteins. It is aseptically filled.

Preparation Note

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water to a final concentration of 100 μg/ml. Adding 0.1% recombinant human serum albumin is recommended. The product is stable for at least 2 years as supplied. After reconstitution, it is recommended to store the protein in working aliquots at –20°C.

Analysis Note

The biological activity of recombinant human IL-6 was tested in culture by measuring its ability to stimulate proliferation of the IL-6 dependent mouse T1165 cells.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Growth Factors in Stem Cell Biology

Stem cell biology researchers use suitable growth factors to trigger proliferation, differentiation and/or migration of stem cells. Embryonic pluripotent stem cells can differentiate into three germ ...
Keywords: Angiogenesis, Cell biology, Cell culture, Growth factors, Stem cell biology

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Peer-Reviewed Papers
15

References

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