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SHC003 Sigma-Aldrich

MISSION® pLKO.1-puro-CMV-TurboGFP Positive Control Plasmid DNA

Green fluorescent protein marker to monitor transduction efficiency

Synonym: MISSION® Control Vectors

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Description

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

TurboBeads is a trademark of TurboBeads LLC

TurboGFP is a trademark of Evrogen Co.

Application

MISSION® pLKO.1-puro-CMV-TurboGFP Positive Control Plasmid DNA has been used to construct GATA2 overexpression vector (oeG2), by removing the GFP cassette of this vector at the 5′ NheI and 3′ PstI cloning sites and replacing it with the full-length GATA2 transcript sequence.

General description

The MISSION® TurboGFP Control Vector is an 8347 base pair lentivirus plasmid vector that contains a gene encoding TurboGFP, under the control of the CMV promoter. The TurboGFP Control Vector is useful as a positive transfection/transduction control in experiments using the MISSION® shRNA library clones. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The TurboGFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What competent cells can I use to propagate the MISSION® Control Vector Purified DNA TurboGFP plasmid, Product SHC003?
We use GC5 cells (identical genotype to DH5α-T1R) to propagate the Mission shRNA plasmids and controls.
How much plasmid DNA do I receive when I order MISSION® Control Vector Purified DNA TurboGFP, Product SHC003?
Each MISSION Control Vector is provided as 10 μg purified plasmid DNA at a concentration of ~500 ng/μL in 10 mM Tris-HCl, pH 8.0, containing 1 mM EDTA.
Can I useMISSION® Control Vector Purified DNA TurboGFP plasmid, Product SHC003, to produce lentiviral particles?
The MISSION® Lentiviral Packaging Mix can be used if you wish to produce virus in your own lab. The product code is SHP001. This product is an optimized formulation of two plasmids expressing the key HIV packaging genes and a heterologous viral envelope gene. The Lentiviral Packaging Mix is designed to be co-transfected along with a compatible lentiviral transfer vector (such as SHC003) into HEK293T cells in order to create high-titer pseudo-typed lentiviral particles used for downstream transduction applications. The procedure is available in the Technical Bulletin for product SHP001.
Can I purchase lentiviral particles produced using MISSION® Control Vector Purified DNA TurboGFP plasmid, Product SHC003, directly from Sigma-Aldrich?
Yes. The product code is SHC003V.
What otherMISSION® shRNA controls are available from Sigma-Aldrich?
A complete list of ourMISSION® controls can be found here.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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SHC002 MISSION® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA, Targets no known mammalian genes
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SHC002V MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, Targets no known mammalian genes
SHC003V MISSION® pLKO.1-puro-CMV-TurboGFP Positive Control Transduction Particles, Green fluorescent protein marker to monitor transduction efficiency
SHC004 MISSION® pLKO.1-puro TurboGFP shRNA Control Plasmid DNA, shRNA sequence targeting tGFP
SHC004V MISSION® pLKO.1-puro TurboGFP shRNA Control Transduction Particles, shRNA sequence targeting tGFP
SHM01 MISSION® ExpressMag® Super Magnetic Kit, Increases transduction efficiency Enhance your lentiviral transduction!
SHM02 MISSION® ExpressMag® 96-Well Magnetic Kit, Increases transduction efficiency Enhance your lentiviral transduction in 96-well plate format!
SHC012 MISSION® pLKO.1-puro-CMV-TagRFP Positive Control Plasmid DNA, Contains a gene encoding TagRFP
SHC014 MISSION® pLKO.1-puro-UbC-TurboGFP Positive Control Plasmid DNA, Contains a gene encoding TurboGFP, under the control of the UbC promoter

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