• USA Home
  • SHC004 - MISSION® pLKO.1-puro TurboGFP shRNA Control Plasmid DNA

EMAIL THIS PAGE TO A FRIEND
SHC004 Sigma-Aldrich

MISSION® pLKO.1-puro TurboGFP shRNA Control Plasmid DNA

shRNA sequence targeting tGFP

Synonym: MISSION® Control Vectors

Purchase

Properties

Related Categories Functional Genomics and RNAi, MISSION Controls in DNA Format, MISSION shRNA, MISSION shRNA Controls, Molecular Biology More...
product line   MISSION®
shipped in   dry ice
storage temp.   −20°C

Description

General description

The MISSION TurboGFP shRNA Control Vector is a 7,087 base pair lentivirus plasmid vector that contains an shRNA sequence targeting TurboGFP. The TurboGFP shRNA Control Vector is useful as a positive knockdown control in experiments using the MISSION TurboGFP positive control vector or in cell lines expressing TurboGFP. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The TurboGFP shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

TurboGFP is a trademark of Evrogen Co.

Application

MISSION® pLKO.1-puro TurboGFP shRNA control plasmid DNA has been used as lentiviral transduction and RNA interference assay.

To see more application data, protocols, vector maps visit sigma.com/shrna.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

recommended

Product #

Description

Comments

Add to Cart

SHC002 MISSION® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA, Targets no known mammalian genes
SHC001V MISSION® pLKO.1-puro Empty Vector Control Transduction Particles, Contains no shRNA insert
SHC003 MISSION® pLKO.1-puro-CMV-TurboGFP Positive Control Plasmid DNA, Green fluorescent protein marker to monitor transduction efficiency
SHC002V MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, Targets no known mammalian genes
SHC003V MISSION® pLKO.1-puro-CMV-TurboGFP Positive Control Transduction Particles, Green fluorescent protein marker to monitor transduction efficiency
SHC004V MISSION® pLKO.1-puro TurboGFP shRNA Control Transduction Particles, shRNA sequence targeting tGFP
SHM01 MISSION® ExpressMag® Super Magnetic Kit, Increases transduction efficiency Enhance your lentiviral transduction!
SHM02 MISSION® ExpressMag® 96-Well Magnetic Kit, Increases transduction efficiency Enhance your lentiviral transduction in 96-well plate format!

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?