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SHC004V Sigma-Aldrich

MISSION® pLKO.1-puro TurboGFP shRNA Control Transduction Particles

shRNA sequence targeting tGFP

Synonym: MISSION® TurboGFP® Control Transduction Particles

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Properties

Related Categories Functional Genomics and RNAi, MISSION Controls in Lentiviral Format, MISSION shRNA, MISSION shRNA Controls, Molecular Biology More...
product line   MISSION®
application(s)   capture ELISA: 106 TU/mL using p24
shipped in   dry ice
storage temp.   −70°C

Description

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.

General description

The MISSION TurboGFP shRNA Control Transduction Particles contain an shRNA sequence targeting TurboGFP. The TurboGFP shRNA Control Particles are useful as a positive knockdown control in experiments using the MISSION TurboGFP positive control vector or in cell lines expressing TurboGFP. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.
The TurboGFP shRNA control transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-TurboGFP shRNA (SHC004). Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

TurboGFP is a trademark of Evrogen Co.

Safety & Documentation

Safety Information

RIDADR 
UN 3245 9
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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SHC002 MISSION® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA, Targets no known mammalian genes
SHC001V MISSION® pLKO.1-puro Empty Vector Control Transduction Particles, Contains no shRNA insert
SHC003 MISSION® pLKO.1-puro-CMV-TurboGFP Positive Control Plasmid DNA, Green fluorescent protein marker to monitor transduction efficiency
SHC002V MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, Targets no known mammalian genes
SHC003V MISSION® pLKO.1-puro-CMV-TurboGFP Positive Control Transduction Particles, Green fluorescent protein marker to monitor transduction efficiency
SHC004 MISSION® pLKO.1-puro TurboGFP shRNA Control Plasmid DNA, shRNA sequence targeting tGFP
SHM01 MISSION® ExpressMag® Super Magnetic Kit, Increases transduction efficiency Enhance your lentiviral transduction!
SHM02 MISSION® ExpressMag® 96-Well Magnetic Kit, Increases transduction efficiency Enhance your lentiviral transduction in 96-well plate format!
SHC016V MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles, Targets no known genes from any species Enhance your lentiviral transduction!

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