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SHC012 Sigma-Aldrich

MISSION® pLKO.1-puro-CMV-TagRFP Positive Control Plasmid DNA

Contains a gene encoding TagRFP

Synonym: MISSION® Control Vectors

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Properties

Related Categories Functional Genomics and RNAi, MISSION Controls in DNA Format, MISSION shRNA, MISSION shRNA Controls, Molecular Biology More...
Quality Level   200
product line   MISSION®
concentration   500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in   dry ice
storage temp.   −20°C

Description

General description

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, we recommend producing self-inactivating replication incompetent viral particles in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The CMV-TagRFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.
The MISSION TagRFP Control Vector is an 8594 base pair lentivirus plasmid vector that contains a gene encoding TagRFP, under the control of the CMV promoter. The CMV-TagRFP Control Vector is useful as a positive control in experiments using the MISSION shRNA library clones.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

TagRFP is a trademark of Evrogen Co.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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