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SHC014 Sigma-Aldrich

MISSION® pLKO.1-puro-UbC-TurboGFP Positive Control Plasmid DNA

Contains a gene encoding TurboGFP, under the control of the UbC promoter

Synonym: MISSION® Control Vectors

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Properties

Related Categories Functional Genomics and RNAi, MISSION Controls in DNA Format, MISSION shRNA, MISSION shRNA Controls, Molecular Biology More...
Quality Level   200
product line   MISSION®
concentration   500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in   dry ice
storage temp.   −20°C

Description

General description

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, we recommend producing self-inactivating replication incompetent viral particles in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The UbC-TurboGFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.
Silencing of the CMV promoter can be a problem in some cell types. For these cells, the ubiquitin promoter (UbC) can be a viable alternative. The MISSION UbC-TurboGFP Control Vector is a 9092 base pair lentivirus plasmid vector that contains a gene encoding TurboGFP, under the control of the UbC promoter. The UbC-Turbo-GFP Control Vector is useful as a positive control in experiments using the MISSION shRNA library clones for applications in which an alternate promoter is necessary.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

TurboGFP is a trademark of Evrogen Co.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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