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SHC014V Sigma-Aldrich

MISSION® pLKO.1-puro-UbC-TurboGFP Positive Control Transduction Particles

Contains a gene encoding TurboGFP, under the control of the UbC promoter

Synonym: MISSION® TurboGFP® Control Transduction Particles

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Properties

Related Categories Functional Genomics and RNAi, MISSION Controls in Lentiviral Format, MISSION shRNA, MISSION shRNA Controls, Molecular Biology More...
product line   MISSION®
application(s)   capture ELISA: 106 TU/mL using p24
shipped in   dry ice
storage temp.   −70°C

Description

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.

When conducting experiments using MISSION shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. Silencing of the CMV promoter can be a problem in some cell types. For these cells, the ubiquitin promoter (UbC) can be a viable alternative. The MISSION UbC-TurboGFP Control Transduction Particles are a positive control to monitor transduction efficiency for applications in which an alternate promoter is necessary. The MISSION UbC-TurboGFP Control Transduction Particles are produced from the sequence-verified 9092 base pair lentivirus plasmid, pLKO.1-puro-UbC-TurboGFP (SHC014).

This construct aids in interpretation of experimental design and results by providing a green fluorescent protein marker for monitoring success in transduction of your cells of interest.

General description

Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

TurboGFP is a trademark of Evrogen Co.

Safety & Documentation

Safety Information

RIDADR 
UN 3245 9
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
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