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SHC201V Sigma-Aldrich

MISSION® TRC2 pLKO.5-puro Empty Vector Control Transduction Particles puro

Contains no shRNA insert

Synonym: MISSION® TurboGFP® Control Transduction Particles

  •  NACRES NA.51

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Properties

Related Categories Functional Genomics and RNAi, MISSION Controls in Lentiviral Format, MISSION shRNA, MISSION shRNA Controls, Molecular Biology More...
product line   MISSION®
shipped in   dry ice
storage temp.   −70°C

Description

General description

The MISSION TRC2 pLKO-puro Control Transduction Particles are produced from the sequence-verified lentiviral plasmid, TRC2 pLKO-puro Control Vector (SHC201). This vector is in the TRC2 pLKO-puro plasmid backbone, which contains the WPRE. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the TRC2 MISSION shRNA library clones. This allows one to examine the effect of transduction on gene expression and interpret the knockdown effect seen with shRNA clones.

Unlike murine-based MMLV or MSCV retroviral systems, lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.

In addition, the lentiviral transduction particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

When conducting experiments using MISSION shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results.

When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Application

Small interfering RNAs (siRNAs) expressed from short hairpin RNAs (shRNAs) are a powerful way to mediate gene specific RNA interference (RNAi) in mammalian cells. The MISSION product line is based on a viral vector-based RNAi library against annotated mouse and human genes. shRNAs that generate siRNAs intracellularly are expressed from amphotropic lentivirus viral particles, allowing screening in a wide range of mammalian cell lines. In these cell lines, MISSION shRNA clones permit rapid, cost efficient loss-of-function and genetic interaction screens.

To see more application data, protocols, vector maps visit sigma.com/shrna.

capture ELISA: 106 TU/mL using p24

Legal Information

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
UN 3245 9
WGK Germany 
3

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
15

References

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SHC201 MISSION® TRC2 pLKO.5-puro Empty Vector Control Plasmid DNA, Contains no shRNA insert
SHC202 MISSION® TRC2 pLKO.5-puro Non-Mammalian shRNA Control Plasmid DNA, Targets no known mammalian genes
SHC203 MISSION® TRC2 pLKO.5-puro-CMV-TurboGFP Positive Control Plasmid DNA, Green fluorescent protein marker to monitor transduction efficiency
SHC204 MISSION® TRC2 pLKO.5-puro TurboGFP shRNA Control Plasmid DNA, shRNA sequence targeting tGFP
SHC202V MISSION® TRC2 pLKO.5-puro Non-Mammalian shRNA Control Transduction Particles, Targets no known mammalian genes
SHC203V MISSION® TRC2 pLKO.5-puro-CMV-TurboGFP Positive Control Transduction Particles, Green fluorescent protein marker to monitor transduction efficiency
SHC204V MISSION® TRC2 pLKO.5-puro TurboGFP shRNA Control Transduction Particles, shRNA sequence targeting tGFP

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