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SHC202V Sigma-Aldrich

MISSION® TRC2 pLKO.5-puro Non-Mammalian shRNA Control Transduction Particles

Targets no known mammalian genes

Synonym: MISSION® TurboGFP® Control Transduction Particles

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Properties

Related Categories Functional Genomics and RNAi, MISSION Controls in Lentiviral Format, MISSION shRNA, MISSION shRNA Controls, Molecular Biology More...
product line   MISSION®
shipped in   dry ice
storage temp.   −70°C

Description

Application

Small interfering RNAs (siRNAs) expressed from short hairpin RNAs (shRNAs) are a powerful way to mediate gene specific RNA interference (RNAi) in mammalian cells. The MISSION product line is based on a viral vector-based RNAi library against annotated mouse and human genes. shRNAs that generate siRNAs intracellularly are expressed from amphotropic lentivirus viral particles, allowing screening in a wide range of mammalian cell lines. In these cell lines, MISSION shRNA clones permit rapid, cost efficient loss-of-function and genetic interaction screens.

To see more application data, protocols, vector maps visit sigma.com/shrna.

General description

This shRNA non-mammalian control was designed using our Turbo GFP sequence and may cause some knockdown of tGFP. For maximum knockdown of tGFP, please refer to SHC004, SHC004V, SHC004H, SHC204, or SHC204V.

The MISSION TRC2 pLKO-puro Non-Target shRNA Control Transduction Particles are produced from the sequence-verified lentiviral plasmid, TRC2 pLKO-puro Non-Target shRNA (SHC202). This vector is in the TRC2 pLKO-puro plasmid backbone, which contains the WPRE. The vector contains an shRNA insert that does not target human or mouse genes, making it useful as a negative control in experiments using the MISSION TRC2 shRNA library clones.

Unlike murine-based MMLV or MSCV retroviral systems, lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.

In addition, the lentiviral transduction particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

When conducting experiments using MISSION shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results.

When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
UN 3245 9
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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SHC201 MISSION® TRC2 pLKO.5-puro Empty Vector Control Plasmid DNA, Contains no shRNA insert
SHC202 MISSION® TRC2 pLKO.5-puro Non-Mammalian shRNA Control Plasmid DNA, Targets no known mammalian genes
SHC203 MISSION® TRC2 pLKO.5-puro-CMV-TurboGFP Positive Control Plasmid DNA, Green fluorescent protein marker to monitor transduction efficiency
SHC204 MISSION® TRC2 pLKO.5-puro TurboGFP shRNA Control Plasmid DNA, shRNA sequence targeting tGFP
SHC201V MISSION® TRC2 pLKO.5-puro Empty Vector Control Transduction Particles puro, Contains no shRNA insert
SHC203V MISSION® TRC2 pLKO.5-puro-CMV-TurboGFP Positive Control Transduction Particles, Green fluorescent protein marker to monitor transduction efficiency
SHC204V MISSION® TRC2 pLKO.5-puro TurboGFP shRNA Control Transduction Particles, shRNA sequence targeting tGFP

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