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SHCLNV Sigma-Aldrich

MISSION® shRNA

Lentiviral Particles

To order shRNA please search for your gene of interest.


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Properties

Related Categories Functional Genomics and RNAi, MISSION shRNA, MISSION shRNA Gene Family Sets, Molecular Biology
application(s)   capture ELISA: 106 TU/mL using p24
shipped in   dry ice
storage temp.   −70°C

Description

General description

Our comprehensive shRNA product offering consists of over 150,000 pre-cloned shRNA constructs targeting more than 15,000 human and 15,000 mouse genes. The library is available in: gene family sets: gene collections related to specific functional classes such as kinases and ion channels; gene taget sets, sets of shRNA clones that target your favorite gene; and individual shRNA clones. Our shRNA and lentiviral manufacturing platforms utilize dedicated laboratories featuring state-of-the-art robotics, liquid handling and laboratory information management systems (LIMS) to provide superior quality and service for your RNAi research needs.

Lentiviral particles are ideal for transducing a wide range of cell lines. VSV-G Psuedotyping of the MISSION® lentiviral particles allows for entry into a wide range of cells. In addition, lentiviral particles stably integrate the shRNA into the genomes of both dividing and non-dividing cell lines. The MISSION bioproduction team regularly produces high quality lentivirus, so that our customers can get straight to their RNAi experiment without wasting precious time on tedious lentiviral production. Our minimum titer is 106 TU/ml. Unlike adenoviral infections that require vast excesses, lentiviral transduction is efficient enought allow for MOI as low as 1.

Application

MISSION® shRNA has been used:
• in transfections and transductions
• in the establishment of stable cell lines expressing shRNA
• in the generation of alpha-actinin-4 (ACTN4) knockdown cell line
• to transduce raw 264.7 cells

Other Notes

To see protocols and application data please visit sigma.com/shrna.

Legal Information

Use of this product is subject to one or more license agreements.

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
UN 3245 9
WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Safe Handling of Lentivirus

Our lentiviral vector systems have been developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication, including eliminating viru...
Keywords: Environmental, Genetic, Recombination, Transfection

Protocols

CRISPR Lentiviral Screening: Antibiotic Selection

A kill curve is a dose-response experiment where cells are subjected to a range of antibiotic concentrations to determine the minimum concentration of antibiotic needed to kill all cells over a desir...
Keywords: Antibiotics, Apoptosis, Gene expression, Transduction

Protocol, FACS Titration of Lentivirus

FACS Analysis of CFU FACS (Fluorescence-Activated Cell Sorting) provides a method for sorting a mixed population of cells into two or more groups, one cell at a time, based on the specific light scat...
Keywords: Enzyme-linked immunosorbent assay, Flow cytometry, Gene expression, Titrations, Transduction

Successful Transduction Using Lentivirus

Successful targeting relies on optimizing key sensitive steps in the process, including lentiviral transduction. Below are some helpful handling and titration tips from our R&D lentiviral experts. As...
Keywords: Antibiotics, Enzyme-linked immunosorbent assay, Gene expression, PAGE, Polymerase chain reaction, Titrations, Transduction

Related Content

MISSION® Lentiviral shRNA Technical Animation [VIDEO]

This video explains how MISSION RNAi (RNA interference) experiments leverage the natural RNAi mechanism. MISSION shRNA reduce the expression of specific target genes by targeting the specific mRNA th...
Keywords: Gene expression

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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