• USA Home
  • SRE0001 - Pepsin from porcine gastric mucosa

EMAIL THIS PAGE TO A FRIEND
SRE0001 Sigma-Aldrich

Pepsin from porcine gastric mucosa

Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥3200 units/mg protein

Synonym: Pepsin A, Pepsin from hog stomach

Purchase

Properties

Related Categories Enzymatic and Chemical Protein Cleavage, Mass Spectrometry, Molecular Biology, Proteases for Cleavage of Proteins, Proteomics More...
Quality Level   400
form   lyophilized powder
specific activity   ≥3200 units/mg protein
mol wt   35 kDa
impurities   salt, essentially free
color   white to off-white
Featured Industry   Diagnostic Assay Manufacturing
shipped in   wet ice
storage temp.   −20°C
Gene Information   pig ... LOC396892(396892)

Description

Packaging

5, 10, 100 g in glass bottle

Application

Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.

Biochem/physiol Actions

Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

Unit Definition

One unit will produce a ΔA280 of 0.001 per min at pH2.0 at 37° C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16mL. Light path = 1cm.)

Analysis Note

Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.

Protein determined by E1%/280

Other Notes

View more information on pepsin at www.sigma-aldrich.com/enzymeexplorer.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
Target organs 
Respiratory system
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
SC6132000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

inhibitor

Product #

Description

Add to Cart

P5318 Pepstatin A, microbial, ≥90% (HPLC)
P7626 Phenylmethanesulfonyl fluoride, ≥98.5% (GC)

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?