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SRE0005 Sigma-Aldrich

Proteinase K from Tritirachium album

Reagents designed and manufactured under cGMP controls suitable for use in an IVD application.

Synonym: Endopeptidase K

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes for Diagnostic Kit Manufacturing, Enzymes, Inhibitors, and Substrates, Molecular Diagnostics More...
Quality Level   GMP
grade   for molecular biology
form   buffered aqueous glycerol solution
mol wt   mol wt 28.93 kDa
concentration   ≥10 mg/mL protein
  ≥800 unit/mL
impurities   ≤500 pg/mg DNA (PicoGreen® assay)
Featured Industry   Diagnostic Assay Manufacturing
foreign activity   DNAse, RNAse, exonuclease, endonuclease, and nickase, none detected
shipped in   wet ice
storage temp.   2-8°C

Description

Biochem/physiol Actions

Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

Unit Definition

One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Application

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

Legal Information

PicoGreen is a registered trademark of Life Technologies

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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