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SRE0022 Sigma-Aldrich

β-Glucuronidase from abalone

Chromatographically purified, lyophilized powder, β-glucuronidase ≥20,000,000 units/g protein

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Properties

Related Categories β-Glucuronidase (beta-Glucuronidase), Analytical and Industrial Enzymes, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Molluskan Source More...
Quality Level   200
form   lyophilized powder
β-glucuronidase activity   ≥20,000,000 units/g protein
secondary activity   ≤4,000,000 units/g protein sulfatase
composition   protein, 10-40%
storage temp.   −20°C

Description

Application

β-Glucuronidase from abalone has been used in enzyme treatment to know the effect of osmotic shock, enzymatic incubation, pulsed electric field, and high shear homogenization on the release of water-soluble proteins and carbohydrates from the green alga Ulva lactuca.

Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine.

Packaging

500000, 1000000, 2000000 units in poly bottle

Biochem/physiol Actions

β-Glucuronidase (GUSB) is a lysosomal enzyme. It degrades glucuronate-containing glycosaminoglycan. β-glucuronidase, obtained from abalone has the ability to hydrolyze samples, that has high analyte concentrations. Deficiency of β-glucuronidase results in mucopolysaccharidosis type VII (MPSVII), which leads to lysosomal storage in the brain.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

Physical form

Supplied as a lyophilized powder containing Trizma® and L-arginine

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
LZ8990000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS. Here we evaluate the hydrolysis efficiency of β-gluc...
Jim Blasberg
Harkewal Singh, Kevin Ray Sigma-Aldrich, St. Louis, MO
Keywords: High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites

Related Content

Analytical Enzyme Beta Glucuronidase

Our β-Glucuronidases are routinely used for the enzymatic hydrolysis of glucuronides from urine1,2, plasma3,4, and other fluids5 prior to analysis by enzyme immunoassay, mass spectrometry, gas chroma...
Keywords: Bacterial conjugations, Cancer, Chromatography, Gas chromatography, High performance liquid chromatography, Immunoassay, Mass spectrometry, PAGE, Purification

Peer-Reviewed Papers
15

References

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