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SRE0062 Sigma-Aldrich

Tris-Borate EDTA Buffer, 10X Concentrate

Reagent designed and manufactured under cGMP controls suitable for use in an IVD application

Synonym: TBE Buffer

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffer Solutions
Quality Level   GMP
form   liquid
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp

Description

Application

Tris-Borate EDTA (TBE) buffer is commonly used in the electrophoresis of nucleic acids in agarose and polyacrylamide gels.

TBE buffer is recommended for resolution of RNA and DNA fragments smaller than 1500 bp.

Physical form

10X TBE buffer stock solution contains 0.89 M Trizma(R) with 0.02 M EDTA adjusted to pH 8.3 with boric acid. Dilute with ultrapure water to 1X.

Notes: If precipitation is present in the 10X concentrated buffer, warm the bottle to 37 °C and mix until completely dissolved prior to dilution.
It is recommended 1X working solutions be filtered through a 0.2 mm filter before use.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Protocols & Articles

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