SRE0065 Sigma-Aldrich

HEPES solution, 1M

Reagent designed and manufactured under cGMP controls suitable for use in an IVD application

Synonym: HEPES Buffer, N-(2-Hydroxyethyl)piperazineN′-(2-ethanesulfonic acid)



Related Categories Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffer Solutions
Quality Level   GMP
form   liquid
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp



HEPES buffer preserves a normal physiological pH environment and is commonly used as a buffer in cell culture applications. HEPES buffer provides no nutritional benefit to cells, but adds extra buffering capacity to the cell culture particularly during experiments performed outside the carbon dioxide incubator.

This 1 M stock solution contains HEPES adjusted to pH 7.35 with sodium hydroxide. Add the concentrated buffer to the cell culture medium to reach the desired concentration (typically 10–25 mM).

Notes: If precipitation is present in the concentrated buffer, gently warm the bottle to 37 °C and mix until completely dissolved prior to dilution.

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis


Certificate of Origin

Protocols & Articles

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