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SRE0066 Sigma-Aldrich

Guanidine hydrochloride solution, 6M

Reagent designed and manufactured under cGMP controls suitable for use in an IVD application

Synonym: Aminoformamidine hydrochloride, GuHCl, Guanidinium chloride, Guanidium chloride



Related Categories Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffer Solutions
Quality Level   GMP
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp



Guanidine hydrochloride is commonly used as a denaturant, because of its ability to break hydrogen bonds between amino acid residues. By breaking these bonds, the 3D conformation of the protein is unfolded and the aqueous solubility of the protein is greatly increased. Once denatured, the protein can be easily reduced, modified, or analyzed, in a variety of procedures. Further processing of the sample can allow the proteins to be analyzed by other common protein analysis methods including mass spectrometry, electrophoresis, and enzymatic digests.
Guanidine hydrochloride has also typically been used for the isolation of RNA, to denature globular proteins, and for protein refolding studies. It can also be used to facilitate the generation of tryptic peptides for analysis of complex protein samples.

Physical form

This product is a ready-to-use 6 M guanidine hydrochloride solution. The 6 M Guanidine hydrochloride solution may be used as a stock solution to prepare solutions of lower concentrations as appropriate.

Note: If precipitation is present in the 6 M buffer, warm the bottle to 37 °C and mix until completely dissolved prior to use.

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis

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Certificate of Origin

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Single- & multi-channel pipettes from BRAND
Protocols & Articles

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