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SRE0082 Sigma-Aldrich

Peroxidase from horseradish

Suitable for manufacturing of diagnostic kits and reagents

Synonym: Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase, Peroxidase from horseradish

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Properties

Quality Level   400
activity   ≥250 units/mg solid (using pyrogallol)
shipped in   wet ice
storage temp.   2-8°C

Description

General description

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

Application

Peroxidase from horseradish has been used in western blotting.

Packaging

5000, 30000, 300000 units in glass bottle

Unit Definition

One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Analysis Note

The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.

Other Notes

View more information on peroxidase at www.sigma-aldrich.com/enzymeexplorer.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

HPLC Analysis of Peroxidase on TSKgel® G3000SWXL

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Peroxidase on TSKgel® G3000SWXL
Keywords: Chromatography, High performance liquid chromatography

Peer-Reviewed Papers
15

References

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