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SRP0104 Sigma-Aldrich

HDAC-3/NCOR2 human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

Synonym: HD3, Histone deacetylase 3, RPD3, SMAP45

  •  NACRES NA.32

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Cytokines and Growth Factors, Cytokines, Growth Factors and Hormones, Deacetylases,
biological source   human
recombinant   expressed in baculovirus infected insect cells
assay   ≥90% (SDS-PAGE)
form   aqueous solution
mol wt   37.6 kDa (NCOR2)
  49.7 kDa (HDAC3)
packaging   pkg of 50 μg
storage condition   avoid repeated freeze/thaw cycles
concentration   >0.02 mg/mL
NCBI accession no.   NM_003883
UniProt accession no.   O15379
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... HDAC3(8841)

Description

General description

Complex of human HDAC3, GenBank Accession No. NM_003883, full length with C-terminal His tag, MW = 49.7 kDa. Human NCOR2, amino acid 395-489, GenBank Accession No. NM_006312, N-terminal ST tag, MW = 37.6 kDa, co-expressed in baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 20 mM glutathione and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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