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SRP0110 Sigma-Aldrich

HDAC-8 human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Synonym: CDA07, HD8, HDACL1, Histone deacetylase 8, Histone deacetylase-like 1

  •  NACRES NA.32

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Cytokines and Growth Factors, Cytokines, Growth Factors and Hormones, Deacetylases,
biological source   human
recombinant   expressed in baculovirus infected insect cells
assay   ≥80% (SDS-PAGE)
form   aqueous solution
potency   ≥0.001 units/mg
mol wt   42.4 kDa
packaging   pkg of 50 μg
storage condition   avoid repeated freeze/thaw cycles
concentration   >0.02 mg/mL
NCBI accession no.   NM_018486
UniProt accession no.   Q9BY41
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... HDAC8(55869)

Description

General description

HDAC8 (histone deacetylase 8) is a class I Zn2+-dependent SMC3 (structural maintenance of chromosomes 3) deacetylase. It is the human ortholog of the yeast Zn2+-dependent deacetylase Hos1.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

HDAC8 (histone deacetylase 8) is a regulator of cohesion protein and is essential for recycling of cohesin during cell cycle. This protein functions on both histone and non-histone substrates, and produces free lysine and acetate by catalyzing the hydrolysis of acetyllysine side chain. Till date, 17 missense mutations in this gene have been identified in patients with Cornelia de Lange Syndrome (CdLS) spectrum disorders, where these mutations have led to partial or complete loss of deacetylase function. This protein is linked with malignant types of neuroblastoma, and its expression in patients with metastatic melanoma is linked with increased survival in the same. Studies show that HDAC8 can abnormally deacetylate and inactivate p53 protein, thus, facilitating leukemia stem cells (LSCs) transformation and maintenance. LSCs are essential for promoting and sustaining acute myeloid leukemia (AML), and HDAC8 inactivation results in normal p53 function in inv(16)(+) AML CD34(+) cells.

Unit Definition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Histone Modification and Chromatin Remodeling

Gene expression is governed by complex mechanisms including transcription factor binding to DNA and coordinated changes in chromatin structure. The primary protein components of chromatin are the his...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 10–16
Keywords: Acetylations, Amplification, Cancer, Cell culture, Chromatin immunoprecipitation, Cloning, DNA purification, Diseases, Epigenetics, Gene expression, Immunoprecipitation, Indicators, Methylations, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Purification, Sequencing, Transcription, Whole genome amplification

Peer-Reviewed Papers
15

References

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