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SRP0112 Sigma-Aldrich

HDAC-10 human

recombinant, expressed in baculovirus infected insect cells, ≥10% (SDS-PAGE)

Synonym: HD10, Histone deacetylase 10

  •  NACRES NA.32

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Cytokines and Growth Factors, Cytokines, Growth Factors and Hormones, Deacetylases,
biological source   human
recombinant   expressed in baculovirus infected insect cells
assay   ≥10% (SDS-PAGE)
form   aqueous solution
mol wt   79 kDa
packaging   pkg of 50 μg
storage condition   avoid repeated freeze/thaw cycles
concentration   >0.02 mg/mL
NCBI accession no.   NM_032019
UniProt accession no.   Q969S8
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... HDAC10(83933)

Description

General description

Human HDAC10, amino acids 1-481, GenBank Accession No. NM_032019, with N-terminal GST tag, MW = 79 kDa, expressed in baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 50% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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