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SRP0113 Sigma-Aldrich

HDAC-11 human

recombinant, expressed in baculovirus infected insect cells, ≥10% (SDS-PAGE)

Synonym: HD11, Histone deacetylase 11

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Cytokines and Growth Factors, Cytokines, Growth Factors and Hormones, Deacetylases,
biological source   human
recombinant   expressed in baculovirus infected insect cells
assay   ≥10% (SDS-PAGE)
form   aqueous solution
mol wt   66 kDa
packaging   pkg of 50 μg
storage condition   avoid repeated freeze/thaw cycles
concentration   >0.02 mg/mL
NCBI accession no.   BC009676
UniProt accession no.   Q96DB2
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... HDAC11(79885)

Description

General description

HDAC-11 (histone deacetylase 11) is a zinc-dependent HDAC. It belongs to the HDAC family of proteins and HDAC class IV. It is highly conserved among various species. The mRNA is present in the brain, skeletal muscle, kidney and testis. The gene is mapped to human chromosome 3p25.
Human HDAC11, full length, GenBank Accession No. BC009676, with N-terminal GST tag, MW = 66kDa, expressed in baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

HDAC-11 (histone deacetylase 11) is responsible for deacetylation of nuclear histone and nonhistone proteins, including transcription factors. HDAC-11 catalyzes the deacetylation of histone H4. It binds to CDT1 (DNA replication factor) and is present with S phase-associated replication origins. HDAC-11 is upregulated in various carcinomas. Absence of it results in cell death and suppresses metabolic activity in colon, prostate, breast and ovarian cancer cell lines.

Unit Definition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 50% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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