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SRP0117 Sigma-Aldrich

Sirtuin 3 human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Synonym: Mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase, SIR2L3, SIRT3, Silent mating type information regulation 2, sir2-like 3

  •  NACRES NA.32

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Properties

Related Categories Deacetylases, Epigenetics, Histone Modifying Enzymes, Molecular Biology, Proteins and Peptides for Epigenetic Research More...
biological source   human
recombinant   expressed in E. coli
assay   ≥70% (SDS-PAGE)
form   aqueous solution
mol wt   59 kDa
packaging   pkg of 100 μg
storage condition   avoid repeated freeze/thaw cycles
concentration   >0.02 mg/mL
NCBI accession no.   NM_012239
UniProt accession no.   Q9NTG7
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... SIRT3(23410)

Description

General description

Sirtuin 3 (SIRT3) is a member of a family of NAD+-dependent protein deacetylases called sirtuins (SIRTs). In mammals, there are seven types of SIRTs (SIRT1 to 7). SIRT 3 is present in mitochondrial matrix.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

Sirtuin 3 (SIRT3) is thought to be involved in metabolism as its expression is suppressed in muscles of diabetic animals and increased in white and brown adipose tissues during calorie restriction (CR). This protein modulates the degree of acetylation and activity of acetyl-coenzyme A synthetase 2, an enzyme thought to be involved in energy production during starvation conditions in mammals. Up-regulation of this gene influences the expression of genes involved in mitochondrial function. During fasting, SIRT3 controls mitochondrial intermediary metabolism and fatty-acid use by deacetylating long-chain acyl coenzyme A dehydrogenase (LCAD). It is an essential modulator of basal ATP, and therefore, overall energy homeostasis.

Unit Definition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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