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SRP0120 Sigma-Aldrich

Sirtuin 6 human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Synonym: NAD-dependent ADP-ribosyltransferase sirtuin-6, SIR2L6, SIRT6, sir2-like 6

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Properties

Related Categories Deacetylases, Epigenetics, Histone Modifying Enzymes, Molecular Biology, Proteins and Peptides for Epigenetic Research More...
biological source   human
recombinant   expressed in E. coli
assay   ≥70% (SDS-PAGE)
form   aqueous solution
mol wt   65 kDa
packaging   pkg of 100 μg
storage condition   avoid repeated freeze/thaw cycles
concentration   >0.02 mg/mL
NCBI accession no.   NM_016539
UniProt accession no.   Q8N6T7
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... SIRT6(51548)

Description

General description

Sirtuin 6 (Sir6) is encoded by the gene mapped to human chromosome 19p13.3, a region linked with development of acute leukemia. Sir 6 is mainly expressed in bone cells and in the ovaries, but it is absent in the bone marrow. Sirt6 is a chromatin-associated protein localized specifically in nucleus.
Human Sirtuin 6, GenBank Accession No. NM_016539), full length with N-terminal ST tag, MW = 65kDa, expressed in Escherichia coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

Sirtuin 6 (Sir6) is a mono-ADP-ribosyltransferase, which catalyzes the transfer of ADP-ribose moiety from NAD to itself and histones. It has been implicated in the regulation of DNA repair, genomic stability and telomere integrity, gene expression, and metabolism. In addition, it also prevents age-related disorders and premature aging. Sir6 plays a vital role in the inhibition of nuclear factor-κB (NF-κB)–mediated inflammatory responses in rheumatoid joints. Hence, Sir6 is considered to be a potent target for treatment of rheumatoid arthritis (RA). Sir6 is also majorly implicated in the regulation of glucose and fat metabolism. Sir6 prevent cardiac hypertrophy in vitro via inhibiting NF-κB-dependent transcriptional activity and this effect is relied on its deacetylase activity.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
15

References

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