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SRP0191 Sigma-Aldrich

Sphingosine-1-phosphate lyase1 Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

Synonym: SGPL1, SP-lyase 1, hSPL

  •  NACRES NA.32

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Lipids in Cell Signaling, Sphingolipids
biological source   human
recombinant   expressed in baculovirus infected insect cells
assay   ≥70% (SDS-PAGE)
form   aqueous solution
mol wt   57.5 kDa
packaging   pkg of 10 μg
storage condition   avoid repeated freeze/thaw cycles
concentration   0.19 mg/mL
NCBI accession no.   NM_003901
UniProt accession no.   O95470
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... SGPL1(8879)

Description

General description

SGPL1 (sphingosine-1-phosphate lyase 1) is an ER (endoplasmic reticulum) enzyme which shows a wide level of tissue expression. This protein is composed of putative 568 amino acids, and has a molecular weight of 63.5kDa. This enzyme is a member of the pyridoxal 5′-phosphate-dependent carbon-carbon lyases, which form a subclass of aldehyde lyases. It is a type III membrane protein, also known as type I membrane protein which lacks a cleavable N-terminal signal sequence. The single transmembrane segment is in proximity to the N-terminal which faces the ER lumen. The catalytic region faces the cytoplasm.

Application

Useful for the study of biochemical function.

Biochem/physiol Actions

SGPL1 (sphingosine-1-phosphate lyase 1) enzyme is involved in the synthesis of long-chain aliphatic aldehydes which occurs during several metabolic processes, such as peroxisomal α-oxidation of 3-methyl-branched fatty acids and 2-hydroxy long-chain fatty acids, microsomal oxidation of long-chain alcohols, prenylated protein degradation in the lysosomes, microsomal degradation of phosphorylated sphingoid bases, attack of plasmalogens by myeloperoxidase (MPO)-derived hypochlorous acid, and degradation of (lyso)plasmalogens in the microsomes.s This protein shows reduced expression in OSCC (oral squamous cell carcinoma), colon and prostate cancers, and this is associated with poor prognosis.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 10% glycerol, 3 mM DTT, 0.5 mM pyridoxal 5′ phosphate monohydrate.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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