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SRP0205 Sigma-Aldrich

MEG-1 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym: Megakaryocyte phosphatase, PTPMEG1, PTPN4, Protein tyrosine phosphatase, non-receptor type 4

  •  NACRES NA.32

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Kinase/Phosphatase Biology, Tyrosine Phosphatase (TP), Tyrosine Phosphatase Biology More...
biological source   human
recombinant   expressed in E. coli
assay   ≥90% (SDS-PAGE)
form   aqueous solution
mol wt   60.3 kDa
packaging   pkg of 20 μg
concentration   >0.02 mg/mL
NCBI accession no.   NM_002830
UniProt accession no.   P29074
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PTPN4(5775)

Description

General description

Protein tyrosine phosphatase, Catalytic domain, (amino acds 637-926) (Gene bank accession number NM_002830) with N-terminal GST tag, MW = 60.3 kDa, expressed in an E. coli expression system.

Application

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

Unit Definition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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