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SRP0213 Sigma-Aldrich

PTP1B (1-321)/PTPN1 Active from mouse

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym: PTPN1, Protein tyrosine phosphatase, non-receptor type 1

  •  NACRES NA.32

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Kinase/Phosphatase Biology, Tyrosine Phosphatase (TP), Tyrosine Phosphatase Biology More...
biological source   mouse
recombinant   expressed in E. coli
assay   ≥90% (SDS-PAGE)
form   aqueous solution
mol wt   63.3 kDa
packaging   pkg of 20 μg
storage condition   avoid repeated freeze/thaw cycles
concentration   >0.02 mg/mL
NCBI accession no.   NM_011201.3
UniProt accession no.   P35821
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PTPN1(5770)

Description

General description

Protein tyrosine phosphatase, Catalytic domain, (amino acids 1-321), with N-terminal GST tag, MW = 63.3 kDa, expressed in an E. coli expression system.

Application

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

Unit Definition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Physical form

Formulation: recombinant proteins were eluted in 25 mM Tris-HCL, 75 mM NaCl, pH 8.0, 0.05% Tween, 10 mM glutathione, 1 mM DTT and 50% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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