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SRP0219 Sigma-Aldrich

CD45 Active human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

Synonym: CD45R, Leukocyte common antigen (LCA), PTPRC, Protein tyrosine phosphatase, receptor type, C, T200 leukocyte common antigen

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Properties

Related Categories CD1 to CD48, CD45, Cell Biology, Cell Signaling and Neuroscience, Cell Surface Molecules,
biological source   human
recombinant   expressed in baculovirus infected insect cells
assay   ≥90% (SDS-PAGE)
form   aqueous solution
mol wt   75 kDa
packaging   pkg of 20 μg
storage condition   avoid repeated freeze/thaw cycles
concentration   >0.02 mg/mL
NCBI accession no.   NM_080922
UniProt accession no.   P08575
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PTPRC(5788)

Description

General description

Protein tyrosine phosphatase, receptor type C (PTPRC) or leucocyte common antigen CD45 is expressed on T-cells and is found in multiple isoforms. The extracellular domain of the protein is predicted to have an amino-terminal mucin-like segment, a cysteine-rich domain and three fibronectin type 3 domains. The gene encoding this protein is localized on human chromosome 1q31.3.

Application

Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.

Biochem/physiol Actions

Protein tyrosine phosphatase, receptor type C (PTPRC) or leucocyte common antigen CD45 acts as a protein tyrosine phosphatase in leucocyte signaling. It has a role in flow cytometric diagnostics in leukemia. By dephosphorylation of Src kinases, the protein acts as a regulator of antigen receptor signaling in T and B cells. As a negative regulator of Janus kinases, CD45 inhibits cytokine receptor signaling.

Unit Definition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Physical form

Formulation: recombinant proteins were eluted in 25 mM Tris-HCL, 75 mM NaCl, pH 8.0, 0.05% Tween, 5 mM DTT and 50% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
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References

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