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SRP0265 Sigma-Aldrich

PDE4C Active from mouse

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

Synonym: Dpde1, Dunce, Phosphodiesterase 4C

  •  NACRES NA.32

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Cyclic Nucleotide Metabolism, Cyclic Nucleotide Metabolism Enzymes, G Proteins and Cyclic Nucleotides More...
biological source   mouse
recombinant   expressed in baculovirus infected insect cells
assay   ≥70% (SDS-PAGE)
form   aqueous solution
mol wt   94 kDa
packaging   pkg of 5 μg
NCBI accession no.   NM_000923
UniProt accession no.   Q08493
shipped in   dry ice
storage temp.   −70°C
Gene Information   mouse ... PDE4C(110385)

Description

General description

Mouse PDE4C (GenBank Accession No. NM_000923), full length, with N-terminal GST tag, MW = 94 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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