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SRP0364 Sigma-Aldrich

mTOR/Raptor/MLST8 human

recombinant, expressed in baculovirus infected Sf9 cells, ≥50% (SDS-PAGE)

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Properties

Related Categories Active Kinases, Cell Biology, Cell Signaling and Neuroscience, Kinase/Phosphatase Biology, Serine/Threonine Kinase Biology,
biological source   human
recombinant   expressed in baculovirus infected Sf9 cells
assay   ≥50% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 137 kDa (mTOR)
  mol wt 150 kDa (Raptor)
  mol wt 37 kDa (MLST8)
packaging   pkg of 10 μg
NCBI accession no.   NM_004958 (mTOR)
  NM_020761 (Raptor)
  NM_022372 (MLST8)
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... MLST8(64223), MTOR(2475), RPTOR(57521)

Description

Application

Recombinant mTOR/Raptor/MLST8 has been used in in vitro caspase reactions.

Biochem/physiol Actions

Mechanistic target of rapamycin (mTOR) modulates cell growth and division by integrating nutrient and mitogen signals. It also modulates the functions of antigen-presenting cells, like dendritic cells and activates T-cells. mTOR is also involved in innate and adaptive immunity. MTOR associated protein, LST8 homolog (MLST8) is a subunit of both the mTOR complex 1 and 2 (mTORC1 and 2). It is crucial for the activation of the mTOR kinase and is a regulator of mTOR pathways. Regulatory associated protein of MTOR complex 1 (RAPTOR) has an important role in modulating cell growth and in energy homeostasis as a scaffolding protein. It takes part in recruiting substrates of mechanistic target of rapamycin (mTOR) to the rapamycin-sensitive mTOR complex 1 (mTORC1). RAPTOR also associates with proteins involved in the pathway and aids in phosphorylating them.

General description

This product (recombinant mTOR/Raptor/MLST8) is a complex of human mechanistic target of rapamycin (mTOR), also known as FRAP1 (GenBank Accession No. NM_004958), amino acids 1362-end with N-terminal FLAG-tag, MW = 137 kDa; human MTOR associated protein, LST8 homolog (MLST8) (GenBank Accession No. NM_022372) amino acids 2-326 (end) with N-terminal His-tag, MW = 37 kDa and human regulatory associated protein of MTOR complex 1 (Raptor) (GenBank Accession No. NM_020761) amino acids 2-1335 (end) with N-terminal His-tag, MW=150 kDa, co-expressed in a Baculovirus-infected Sf9 cell expression system.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis


Milli-Q® Water Purification Solutions
Protocols & Articles
Peer-Reviewed Papers
15

References

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